PAK4/5/6 (phospho-S474) polyclonal Antibody | BS4854

PAK4/5/6 (phospho-S474) polyclonal Antibody | BS4854 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IHC ICC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Background: PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-PAK4/5/6 (S474) polyclonal Antibody detects endogenous levels of PAK4, PAK5 and PAK6 only when phosphorylated at serine 474, 602, or 560, respectively. The Antibody does not cross-react with phosphorylated PAK1, PAK2 or PAK3.

Molecular Weight: ~ 72 kDa (PAK4), 82 kDa (PAK6), 90 (PAK7)

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7; PAK-7; PAK7; KIAA1264

Immunogen: Synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Serine 474.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Regulation of Microtubule Dynamics,