PAPOLA polyclonal Antibody | BS61818

PAPOLA polyclonal Antibody | BS61818 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB

Application Range: WB: 1:500~1:1000

Background: Polyadenylation of the 3' ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction occurs in two distinct steps: endoribonucleolytic cleavage of the pre-RNA at the poly (A) site, followed by synthesis of the poly (A) tail at the 3' end of the up-stream cleavage product. The poly (A) polymerase (PAP) is required for the adenosine addition reaction. Western blot analysis reveals three PAPs, namely PAP-α, PAP-β and PAP-γ, demonstrating different molecular masses in HeLa cell extracts. The amino-terminal region of PAP is required for nonspecific polymerase activity, while both the amino and carboxy termini are required for specific polymerase activity. Additionally, PAP contains a functional ribonucleoprotein-type RNA binding domain (RBD) that is responsible for primer binding.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PAPOLA pAb detects endogenous levels of PAPOLA protein.

Molecular Weight: ~ 100 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Poly (A) polymerase alpha; PAP-alpha; Polynucleotide adenylyltransferase alpha; PAPOLA; PAP

Immunogen: Synthetic peptide, corresponding to Human PAPOLA.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: