Paralemmin3 Antibody | 6449

Paralemmin3 Antibody | 6449 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (79%)

Immunogen: Paralemmin3 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Paralemmin3.
The immunogen is located within amino acids 50 - 100 of Paralemmin3.

Research Area: Innate Immunity

Tested Application: E, WB, ICC, IF

Application: Paralemmin3 antibody can be used for detection of Paralemmin3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of Paralemmin3 are known to exist; this antibody will only detect the longer isoform

Positive Control 1: Cat. No. 1219 - MCF7 Cell Lysate

Positive Control 2: Cat. No. 17-012 - MCF7 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Paralemmin3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Paralemmin3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Paralemmin3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Paralemmin3 Antibody: Paralemmin-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Paralemmin3 Antibody: Paralemmin3 is a recently identified protein that associates with the single immunoglobulin IL-1 receptor-related molecule (SIGIRR) , a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Silencing of Paralemmin3 expression by RNAi inhibited the release of inflammatory cytokines in A549 cells after stimulation with lipopolysaccharide (LPS) , suggesting Paralemmin3 may function as an adaptor in the LPS-TLR4 signaling pathway.