PAX6 Antibody [PAX6/498] | 33-183

PAX6 Antibody [PAX6/498] | 33-183 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat, Chicken

Homology: N/A

Immunogen: Recombinant human PAX6 protein was used as the immunogen for this antibody.

Research Area: Cell Cycle, Neuroscience, Signal Transduction

Tested Application: WB, Flow, IF

Application: Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G purified antibody

Clonality: Monoclonal

Clone: PAX6/498

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: PAX genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the PAX6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. PAX6 is involved in other anterior segment malformations besides aniridia, such as Peters��� anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The PAX6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human PAX6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.