PCNA, human recombinant | 4835

Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase- to play a fundamental role in the initiation of cell proliferation. Recombinant PCNA was expressed with a 6x His tag in sf9 insect cells and purified by proprietary chromatographic techniques.

4835 | PCNA human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367,

Alternates names: Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367,

Taglines: A processivity factor for DNA polymerase

NCBI Gene ID #: 4803

NCBI Gene Symbol: NGF

Gene Source: Human

Accession #: P01138

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 90%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): Not available at this time

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 29.8 kDa

Concentration: 5 µg/5 l

Appearance: Liquid

Physical form description: Sterile filtered and (5 µg/5 µl) in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA, and 20% Glycerol.

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). This can further be diluted to other aqueous buffers.

Amino acid sequence: Recombinant human ProNGF produced in E. coli is a non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met and having a molecular mass of 25 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Pro-His-Ser.