PD-1/PDCD1, Human Recombinant | P1024

Programmed death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN- by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN- secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. It can also be used as an immunogen, as a protein standard, or in cell biology research applications.

P1024 | PD-1/PDCD1 Human Recombinant DataSheet

Biomolecule/Target: PD-1/PDCD1

Synonyms: PD, PD 1, PD 1 protein, PD 1 recombinant protein, human PD 1 protein, human recombinant PD 1 protein, human recombinant PD 1, human PD 1, recombinant PD 1, , PD, PD-1, PD-1 protein, PD-1 recombinant protein, human PD-1 protein, human recombinant PD-1 protein, human recombinant PD-1, human PD-1, recombinant PD-1, PD, PD1, PD1 protein, PD1 recombinant protein, human PD1 protein, human recombinant PD1 protein, human recombinant PD1, human PD1, recombinant PD1, PD1, PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l, October-RP-14, PDCD, PDCD 1, PDCD 1 protein, PDCD 1 recombinant protein, human PDCD 1 protein, human recombinant PDCD 1 protein, human recombinant PDCD 1, human PDCD 1, recombinant PDCD 1, PDCD, PDCD-1, PDCD-1 protein, PDCD-1 recombinant protein, human PDCD-1 protein, human recombinant PDCD-1 protein, human recombinant PDCD-1, human PDCD-1, recombinant PDCD-1, PDCD, PDCD1, PDCD1 protein, PDCD1 recombinant protein, human PDCD1 protein, human recombinant PDCD1 protein, human recombinant PDCD1, human PDCD1, recombinant PDCD1, PDCD1

Alternates names: GSK, Glycogen synthase kinase-3 (GSK-3)

Taglines: Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN- by suppressing the activation and transduction of PI3K/AKT pathway.

NCBI Gene ID #: 2932

NCBI Gene Symbol: GSK3B

Gene Source: Human

Accession #: P49841

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): Activity of GSK-3 was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3 in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM -glycerol phosphate, 12 mM MgCl, 2 mM DTT, 0.1 M Na3VO4, and 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation of Tau by the recombinant GSK-3 was determined by Western blot using a phospho-Tau antibody.

Biological activity:

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 53.0-56.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 2 mg/ml in 0.1 M NH4CO3

Reconstitution Instructions: Reconstitute to 1 mg/ml in PBS containing 15% glycerol. The solution should be stored at –70°C, stable for up to 3 months.

Amino acid sequence: N/A