PD-L1 Antibody | 46-156

PD-L1 Antibody | 46-156 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: N/A

Immunogen: The immunogen for this antibody is: CKKQSDTHLEET

Research Area: Cancer

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human Heart lysates. The observed molecular weight corresponds to glycosylation (calculated MW of 33.3kDa according to NP_054862.1) . An additional band was also consistently observed at 55kDa, and was successfully blocked by incubation with the immunizing peptide. Preliminary testing also showed the 37+55kDa bands in lysates of cell lines, A549, Daudi, HeLa, HepG2 and Jurkat Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Placenta shows membranous staining of cytotrophoblasts. Recommended concentration: 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognize reported isoforms a and b (NP_054862.1; NP_001254635.1) only.

Positive Control 1: Cat. No. 1301 - Human Heart Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: CD274, CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635, CD274 molecule, MGC142294, MGC142296

User Note: N/A

BACKGROUND: N/A