PDGF-BB, murine recombinant | 4489

Recombinant mouse Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a homodimeric, non-glycosylated, polypeptide of 109 amino acids and having a molecular mass of 24.4 kDa.

4489 | PDGF-BB murine recombinant DataSheet

Biomolecule/Target: PDGF-BB

Synonyms: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor polypeptide, Becaplermin.

Alternates names: soluble IL-4 receptor alpha, CD124

Taglines: A growth factor that regulates cell growth and division

NCBI Gene ID #: 3566

NCBI Gene Symbol: IL4RA

Gene Source: Human

Accession #: P24394

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of 2 x 105 units/mg.

Biological activity: Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of 2 x 105 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~24 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS pH 7.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to - 80°C

Amino acid sequence: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH