PDIA1 Antibody | 8213

PDIA1 Antibody | 8213 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Rabbit: (100%) , Bovine: (100%) , Chicken: (88%)

Immunogen: PDIA1 antibody was raised against a 17 amino acid peptide near the center of human PDIA1.
The immunogen is located within amino acids 190 - 240 of PDIA1.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: PDIA1 antibody can be used for detection of PDIA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: PDIA1 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1468 - Rat Small Intestine Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 54 kDa
Observed: 54 kDa

Validation: N/A

Isoform: N/A

Purification: PDIA1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PDIA1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PDIA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Protein disulfide isomerase family A member 1, DSI, GIT, PDI, PHDB, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PDIA1 (protein disulfide isomerase family A member 1) is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen (1, 2) . PDIA1 is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds (3) . Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (4) .