Additional Information
Size: |
100 µl |
Target: |
PDK1 (pSer241) |
Conjugate: |
Dylight 350 |
Research Area: |
Cell Signaling | Cytoskeleton | Microfilaments | Actin | Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases |
Alternative Name: |
PDK1 Antibody, PDPK1 Antibody, PDPK1_HUMAN Antibody, PDPK2 Antibody, PkB kinase Antibody, PkB kinase like gene 1 Antibody, PkB like 1 Antibody, PRO0461 Antibody, Protein kinase Antibody3 phosphoinositide dependent protein kinase 1 Antibody, 3-phospho |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
A phospho-specific peptide corresponding to residues surrounding Ser241 of human PDK1 (AA238-244) |
Immunogen Species: |
Human |
Applications: |
WB, AM |
Host: |
Rabbit |
Isotype: |
N/A |
Species Reactivity: |
Human |
Antibody Dilution: |
WB (1:250); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
Concentration: |
N/A |
Specificity: |
Detects ~63kDa. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:250 dilution of SPC-1050 was sufficient for detection of PDK1 (pSer241) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Cellular Localization: |
Cytoplasm, Nucleus, Cell Membrane, Cell Junction, Focal Adhesion |
Tissue Specificity: |
Expressed ubiquitously with highest expression in blood vessels and the brain. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast. |
Accession Number: |
NP_002601 |
Gene ID: |
5170 |
Swiss-Prot: |
O15530 |
Field of Use: |
For in vitro research use only. |