Periostin/OSF-2, murine recombinant | 4205

Periostin was originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor 1 (TGF-1) and bone morphogenetic protein (BMP-2). Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. ’s Mouse periostin (aa 24-783) (isoform 5) is fused at the C-terminus to a FLAG®-tag.

4205 | Periostin/OSF-2 murine recombinant DataSheet

Biomolecule/Target: Periostin/OSF-2

Synonyms: OSF-2, Periostin, Osteoblast Specific Factor 2, PN OSF-2,PDLPOSTN, POSTN, MGC119510, MGC119511, PN, RP11-412K4.1.

Alternates names: OSF-2, Periostin, Osteoblast Specific Factor 2, PN OSF-2,PDLPOSTN, POSTN, MGC119510, MGC119511, PN, RP11-412K4.1.

Taglines: A ligand for integrins

NCBI Gene ID #: 3627

NCBI Gene Symbol: CXCL10

Gene Source: Mouse

Accession #: P02778

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: <0.1 EU/g

Activity (Specifications/test method): N/A

Biological activity: The ED50 of rHu CXCL10 as determined by its ability to chemoattract human CXCR3 transfected BaF3 mouse proB cells was 0.02-0.06 g/mL.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 85 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Solid (Lyophilized from PBS)

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml). This can further be diluted to other aqueous buffers.

Amino acid sequence: Recombinant human CXCL10/IP-10/CRG-2 is produced by E.coli transformed with a plasmid contains sequence (Val22-Pro98) of human CXCL10/IP-10/CRG-2 (Uniprot Entry: P02778) fused with a polyhistidine tag at the C-terminus. The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Leu-Ser-Arg.