PGP, human recombinant | P1190

Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1190 | PGP human recombinant DataSheet

Biomolecule/Target: PGP

Synonyms: Phosphoglycolate phosphatase, Glycerol-3-phosphate phosphatase, G3PP, Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, AUM

Alternates names: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.

Taglines: Plays a role in regulating glucose, lipid and energy metabolism

NCBI Gene ID #: 16171

NCBI Gene Symbol: IL17A

Gene Source: Mouse

Accession #: Q62386

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Measured by its ability to induce IL-6 production by NIH 3T3 cells.

Biological activity: Measured by its ability to induce IL-6 production by NIH 3T3 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 30 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAA