PGR (phospho-S400)  polyclonal Antibody | BS64224

PGR (phospho-S400)  polyclonal Antibody | BS64224 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Rat,Mouse

Application: WB

Application Range: WB: 1:500~1:1000

Background: Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B. Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription. The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms. Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2. Mutation of Ser190 results in decreased activity of PR, suggesting that the phosphorylation at Ser190 may be critical to its biological function.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PGR (Phospho-S400) polyclonal Antibody detects endogenous levels of PGR protein only when phosphorylated at Ser400.

Molecular Weight: ~ 98 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3

Immunogen: Synthetic phosphopeptide derived from human PGR around the phosphorylation site of Serine 400.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: