Phospho-ARHGAP35 (Y1105) antibody [APR32955G]

Phospho-ARHGAP35 (Y1105) antibody [APR32955G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1090-1135 amino acids from human.

Cellular Localisation: Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q91YM2}. Cytoplasm {ECO:0000250|UniProtKB:Q91YM2}. Nucleus Cell membrane {ECO:0000250|UniProtKB:Q91YM2}. Note=In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin. {ECO:0000250|UniProtKB:Q91YM2}

Molecular Weight: 170514

Clone: Polyclonal

Gene Name: ARHGAP35 (HGNC:4591)

Gene ID: 2909

Function: Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085) . Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492) . This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492) . Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity) . Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity) . Transduces SRC- dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity) . Modulates Rho GTPase- dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity) . During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity) . Represses transcription of the glucocorticoid receptor by binding to the cis- acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621) .

Summary: Tissue Location: Detected in neutrophils (at protein level) .

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:500

Synonyms: Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, GRF-1, Rho GAP p190A, p190-A, ARHGAP35, GRF1, GRLF1, KIAA1722