Phospho-Endophilin (Y80) Antibody [APR09160G]

Phospho-Endophilin (Y80) Antibody [APR09160G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This Endophilin Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y80 of human Endophilin.

Cellular Localisation: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasmic vesicle, autophagosome membrane. Midbody. Note=Association with the Golgi apparatus depends on the cell type (By similarity) . Following starvation colocalizes with ATG5 and LC3 autophagy-related protein (s) on autophagosomal membranes (PubMed:17891140) . {ECO:0000250, ECO:0000269|PubMed:17891140}

Molecular Weight: 40796

Clone: Polyclonal

Gene Name: SH3GLB1

Gene ID: 51100

Function: May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144) . Required for coatomer-mediated retrograde transport in certain cells (By similarity) . May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418) . Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588) . Isoform 1 acts proapoptotic in fibroblasts (By similarity) . Involved in caspase- independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140) . Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542) . Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123) . Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity) .

Summary: Tissue Location: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: DB

Dilution: DB--1:500

Synonyms: Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1, SH3GLB1, KIAA0491