Phospho-EP300 (S89) Antibody [APR09163G]

Phospho-EP300 (S89) Antibody [APR09163G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300.

Cellular Localisation: Cytoplasm. Nucleus. Chromosome. Note=Localizes to active chromatin: Colocalizes with histone H3 acetylated and/or crotonylated at 'Lys-18' (H3K18ac and H3K18cr, respectively) (PubMed:25818647) . In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus (PubMed:12929931) .

Molecular Weight: 264161

Clone: Polyclonal

Gene Name: EP300

Gene ID: 2033

Function: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (PubMed:23415232, PubMed:23934153, PubMed:8945521) . Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation (PubMed:23415232, PubMed:23934153, PubMed:8945521) . Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (PubMed:23911289) . Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2 (PubMed:12929931, PubMed:16762839, PubMed:18722353) . Acetylates 'Lys- 131' of ALX1 and acts as its coactivator (PubMed:12929931) . Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (PubMed:18722353) . Acetylates HDAC1 leading to its inactivation and modulation of transcription (PubMed:16762839) . Acetylates 'Lys-247' of EGR2 (By similarity) . Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (PubMed:12586840) . Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity (PubMed:15890677) . Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (PubMed:12402037) . Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (PubMed:14645221) . Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:16617102) . Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178) . Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902) . Acetylates MEF2D (PubMed:21030595) . Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990) . Acetylates GABPB1, impairing GABPB1 heterotetramerization and activity (By similarity) . In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E) -butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA), 2- hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA), lactoyl-CoA or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation, 2-hydroxyisobutyrylation, lactylation or propionylation, respectively (PubMed:17267393, PubMed:25818647, PubMed:29775581, PubMed:31645732) . Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25818647) . Histone crotonyltransferase activity is dependent on the concentration of (2E) -butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (2E) -butenoyl-CoA (crotonyl-CoA) concentration is low (PubMed:25818647) . Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:17267393) . Catalyzes histone lactylation in macrophages by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription (PubMed:31645732) . Acts as a protein-lysine 2- hydroxyisobutyryltransferase; regulates glycolysis by mediating 2- hydroxyisobutyrylation of glycolytic enzymes (PubMed:29775581) . Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (PubMed:25514493) . Acetylates PCK1 and promotes PCK1 anaplerotic activity (PubMed:30193097) . Acetylates RXRA and RXRG (PubMed:17761950) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: DB, IHC-P

Dilution: DB--1:500 IHC-P--1:100~500

Synonyms: Histone acetyltransferase p300, p300 HAT, E1A-associated protein p300, EP300, P300