Phospho LIMK1/2 (Thr508/505) Antibody | A1965

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

A1965 | Phospho LIMK1/2 (Thr508/505) Antibody DataSheet

Antibody Target: Phospho LIMK1/2 (Thr508/505)

Target Alternative Name: LIMK; LIM domain kinase 1; LIMK-1

Tag Line: Affinity purified rabbit polyclonal antibody to detect phosphorylation of LIMK1/2 at Thr508/505 in human, rat, bovine and chicken samples

Category: Primary antibody

Host: Rabbit

Isotype: IgG

Species Reactivities: Bovine, Rat, Chicken, Human

Immunogen Sequence: KLH-conjugated synthetic peptide targeting a sequence within the C-term region of human LIMK1/2

Accession #: P53667

Gene ID: 3984

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide

Purification: Affinity purified

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting

Positive Control:

Application And Usages: WB 1:500 - 1:1000, IHC 1:100 - 1:200, IF 1:100 - 1:500

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: True

Immunofluorescence Verified: True

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: False

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False