Phospho-PKR (Thr446) monoclonal Antibody | BS65508

Phospho-PKR (Thr446) monoclonal Antibody | BS65508 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: IHC-P

Application Range: IHC-P=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

Background: PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-PKR (Thr446) monoclonal Antibody detects endogenous levels of Nrf2 protein only when phosphorylated at Thr446.

Molecular Weight: ~67kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: PKR (T446) ; PKR (phospho T446) ; p-PKR (phospho T446) ; PKR (Phospho-Thr446) ; double-stranded RNA-dependent Protein Kinase; interferon-induced, double-stranded RNA-activated protein kinase isoform a; protein kinase, interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; protein kinase RNA-activated; interferon-inducible RNA-dependent protein kinase; EIF2AK2; EIF2AK1; MGC126524; PKR; PRKRv; E2AK2_HUMAN.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PKR around the phosphorylation site of Thr446:KR (p-T) RS

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: affinity purified by Protein A

Pathway: hippo Signaling,