Phospho PTEN (Ser370) Antibody | A1952

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

A1952 | Phospho PTEN (Ser370) Antibody DataSheet

Antibody Target: Phospho PTEN (Ser370)

Target Alternative Name: MMAC1; TEP1; Phosphatidylinositol 3;4;5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog

Tag Line: Affinity purified rabbit polyclonal antibody to detect phosphorylation of PTEN at Ser370 in human, mouse, bovine, dog, monkey, pig and zebrafish samples

Category: Primary antibody

Host: Rabbit

Isotype: IgG

Species Reactivities: Bovine, Porcine, Dog, Monkey, Zebrafish, Mouse, Human

Immunogen Sequence: KLH-conjugated synthetic peptide targeting a sequence within the C-term region of human PTEN

Accession #: P60484

Gene ID: 5728

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide

Purification: Affinity purified

Application: Immunohistochemistry (IHC), Western Blotting

Positive Control:

Application And Usages: IHC 1:100 - 1:200, WB 1:500 - 1:1000

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: True

Immunofluorescence Verified: False

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: False

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False