Phospho RPS6 (Ser235) Antibody | A1960

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

A1960 | Phospho RPS6 (Ser235) Antibody DataSheet

Antibody Target: Phospho RPS6 (Ser235)

Target Alternative Name: 40S ribosomal protein S6; Phosphoprotein NP33

Tag Line: Affinity purified rabbit polyclonal antibody to detect phosphorylation of RPS6 at Ser235 in human, mouse, rat, bovine, chicken, monkey, sheep and zebrafish samples

Category: Primary antibody

Host: Rabbit

Isotype: IgG

Species Reactivities: Bovine, Sheep, Zebrafish, Rat, Chicken, Monkey, Mouse, Human

Immunogen Sequence: KLH-conjugated synthetic peptide targeting a sequence within the C-term region of human RPS6

Accession #: P62753

Gene ID: 6194

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide

Purification: Affinity purified

Application: Immunofluorescence (IF), Western Blotting

Positive Control:

Application And Usages: WB 1:500 - 1:1000, IF 1:100 - 1:500

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: False

Immunofluorescence Verified: True

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: False

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False