Phosphogluconate dehydrogenase, human recombinant | P1051

PGD (Phosphogluconate dehydrogenase), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

P1051 | Phosphogluconate dehydrogenase human recombinant DataSheet

Biomolecule/Target: PGD

Synonyms: 6PGD, PGD

Alternates names: IL20, NIRF

Taglines: An enzyme involved in the pentose phosphate shunt, major source of NADPH.

NCBI Gene ID #: 27190

NCBI Gene Symbol: IL17B

Gene Source: Human

Accession #: Q9UHF5

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to induce IL-8 production in human PBMCs using a concentration range of 10.0-100.0 ng/ml. Please note: Results may vary with PBMC donors.

Biological activity: Determined by its ability to induce IL-8 production in human PBMCs using a concentration range of 10.0-100.0 ng/ml. Please note: Results may vary with PBMC donors.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 36.6 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS, pH 7.5.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F