Phosphothreonine | AS18 4171

Phosphothreonine | AS18 4171 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated phosphothreonine

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.

Format: Liquid

Tested Application: ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)

Related Products: Plant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)

Molecular weight: N/A

Confirmed Reactivity: Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: 2 µg/ml of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.

Background: Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.

Reconstitution: N/A

Storage: Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0, 01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Signal transduction

Research Area: Cell Signaling