PHPT1, human recombinant | P1181

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1181 | PHPT1 human recombinant DataSheet

Biomolecule/Target: PHPT1

Synonyms: bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, 14 kDa phosphohistidine phosphatase

Alternates names: C16orf77

Taglines: Enzyme that exhibits phosphohistidine phosphatase activity

NCBI Gene ID #: 146433

NCBI Gene Symbol: IL34

Gene Source: Human

Accession #: Q6ZMJ4

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Measured by its ability to induce secretion of MCP-1 from PBMCs.

Biological activity: Measured by its ability to induce secretion of MCP-1 from PBMCs.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 52.5 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Citric Acid, pH 3.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: NEPLEMWPLT QNEECTVTGF LRDKLQYRSR LQYMKHYFPI NYKISVPYEG VFRIANVTRL QRAQVSEREL RYLWVLVSLS ATESVQDVLL EGHPSWKYLQ EVETLLLNVQ QGLTDVEVSP KVESVLSLLN APGPNLKLVR PKALLDNCFR VMELLYCSCC KQSSVLNWQD CEVPSPQSCS PEPSLQYAAT QLYPPPPWSP SSPPHSTGSV RPVRAQGEGL LPHHHHHHHH