Phy | Phytochrome (clone Oat-25) | AS20 4500

Phy | Phytochrome (clone Oat-25) | AS20 4500 | Gentaur UK, US & Europe Distribution

Immunogen: Phytochrome

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG

Purity: Immunoglobulin Protein G purified, in PBS pH 7.4, 150 mM NaCl, 0.05 % of sodium azide.

Format: Liquid

Tested Application: ELISA (ELISA), Competitive ELISA, Immunoprecipiation (IP), Western blot (WB)

Related Products: developmental biology antibody collection

Recommended Dilutions: assay dependent

Molecular weight: 124 kDa

Confirmed Reactivity: Avena sativa, Pisum sativum

Predicted Reactivity: graminae, fabaceaeSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Epitope recognized by this antibody is located at N-terminus of phytochrome, This domain undergoes conformational change when phytochrome is interconverted between the red and far-red absorbing forms

Background: Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr), 4 and an active far-red-absorbing form (Pfr) . When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.

Reconstitution: N/A

Storage: Store at -20°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Photomorphogenesis

Research Area: Plant Biology