PiBlue Phosphate Assay Kit

Application: For sensitive and high-throughput phosphate determination.

Key Features:

• Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits.
• High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 μM and 50 μM phosphate.
• Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes.
• Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers.
• Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems

Method: OD620nm (malachite green)

Samples: Enzyme reactions with free phosphate releases

Species: All

Size: 500 tests

Detection Limit: 0.4 μM

Shelf Life: 12 months

More Details:

The PiBlue™ Phosphate Assay Kit is based on a proprietary formulation of the malachite green dye. The PiBlue™ reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life.

The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high-throughput screening of enzyme inhibitors.