PIF3 | Phytochrome interacting factor 3 (goat antibody) | AS16 3954

PIF3 | Phytochrome interacting factor 3 (goat antibody) | AS16 3954 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana PIF3, UniProt: O80536, TAIR: AT1G09530

Host: Goat

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS16 3157 | Anti-PIF4 | Phytochrome interacting factor 4, rabbit antibodiesAS16 3955 | Anti-PIF4 | Phytochrome interacting factor 4, goat antibodiesAS12 2112 | Anti-PIF5 | PHYTOCHROME INTERACTING FACTOR 5, rabbit antibodiesPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 57 | ca, 65 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Cardamine hirsuta Species of your interest not listed? Contact us

Not reactive in: Nicotiana attenuata

Additional Information: PIF3 protein runs at higher MW than expected, as observed previously (Al-Sady et al. 2006) . PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light) .

Background: PIF3 (Phytochrome interacting factor 3) is a transcription factor which acts positively in the phytochrome signaling pathway. It activates transcription by binding to the G box. Subcellular localization is nucleus. Alternative names:Basic helix-loop-helix protein 8, AtbHLH8, bHLH8, Phytochrome-associated protein 3, Phytochrome-interacting factor 3, Transcription factor EN 100, EN100, bHLH transcription factor bHLH008, PAP3, PHYTOCHROME-ASSOCIATED PROTEIN 3, POC1, PHOTOCURRENT 1, ABI1, ABA INSENSITIVE 1, AtABI1.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G09530

Category: Transcription regulation

Research Area: Plant Biology