Pig Diamine oxidase (DAO) ELISA Kit | AE58939PI

Pig Diamine oxidase (DAO) ELISA Kit | AE58939PI | Gentaur UK, US & Europe Distribution

Species Reactivity: Pig (Sus scrofa; Porcine)

Abbreviation: DAO

Alternative Name: DAAO; DAMOX; MGC35381; OXDA;

Application: ELISA

Range: 3.12-200 ng/mL

Sensitivity: 1.17 ng/mL

Intra-Assay: ≤5.3%

Inter-Assay: ≤7.1%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate DAO in samples. An antibody specific for DAO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDAO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: On the basis of its primary structure, the amiloride-binding protein (EC 1.4.3.6) is 713 amino acids long, with a 19-amino acid signal peptide. Expressed in cultured cells, the mRNA yields a glycoprotein that binds amiloride and amiloride analogs with affinities similar to the amiloride receptor associated with the apical Na+ channel in pig kidney membranes and is immunoprecipitated with monoclonal antibodies raised against pig kidney amiloride-binding protein. Barbry et al. (1990) pointed out that amiloride-sensitive Na+ channels are also present in airway epithelia, where they play an important role in fluid secretion. Amiloride inhibits the excessive absorption of Na+ and liquid that takes place in airway epithelia of patients with cystic fibrosis, and amiloride aerosol therapy has been tried for the treatment of lung disease in CF.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .