855

Pig EGF (Epidermal Growth Factor) ELISA Kit | ELK5718

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SKU:
855-ELK5718-GEN
NULL666.00 - NULL813.00

Description

Pig EGF (Epidermal Growth Factor) ELISA Kit | ELK5718 | Gentaur UK, US & Europe Distribution

Alternative Names: URG; Beta-Urogastrone; Pro-epidermal growth factor

Species: Pig

Assay Type: Sandwich

Sensitivity: 5.7 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor (EGF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epidermal Growth Factor (EGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epidermal Growth Factor (EGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epidermal Growth Factor (EGF) in the samples is then determined by comparing the OD of the samples to the standard curve.

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