Pig PAG2 (Pregnancy associated glycoprotein 2) ELISA Kit | ELK8656

Pig PAG2 (Pregnancy associated glycoprotein 2) ELISA Kit | ELK8656 | Gentaur UK, US & Europe Distribution

Alternative Names: PAG2; Pregnancy associated glycoprotein 2

Species: Pig

Assay Type: Sandwich

Sensitivity: 0.129 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Pregnancy diagnosis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy associated glycoprotein 2 (PAG2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pregnancy associated glycoprotein 2 (PAG2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy associated glycoprotein 2 (PAG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnancy associated glycoprotein 2 (PAG2) in the samples is then determined by comparing the OD of the samples to the standard curve.