Pig Trypsin (TRY) ELISA Kit | AE13185PI

Pig Trypsin (TRY) ELISA Kit | AE13185PI | Gentaur UK, US & Europe Distribution

Species Reactivity: Pig (Sus scrofa; Porcine)

Abbreviation: TRY

Alternative Name: N/A

Application: ELISA

Range: 1.56-100 ng/mL

Sensitivity: 0.62 ng/mL

Intra-Assay: ≤5.8%

Inter-Assay: ≤9.2%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TRY in samples. An antibody specific for TRY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRY bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. The process is commonly referred to as trypsin proteolysis or trypsinisation and proteins that have been digested/treated with trypsin are said to have been trypsinized.Trypsin is secreted into the duodenum, where it acts to hydrolyse peptides into their smaller building blocks, namely amino acids (these peptides are the result of the enzyme pepsin breaking down the proteins in the stomach) . This is necessary for the uptake of protein in the food as though peptides are smaller than proteins, they are still too big to be absorbed through the lining of the ileum. Trypsin catalyses the hydrolysis of peptide bonds.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .