Additional Information
Size: |
100 µl |
Target: |
PIK3R1 + PIK3R3 (pTyr467/199) |
Conjugate: |
Biotin |
Research Area: |
Cell Signaling | Lipid Signaling | Lipid Kinases | Cancer | Immunology | Innate Immunity | TLR Signaling |
Alternative Name: |
PIK3R1 + PIK3R3 Antibody, PI3-kinase p85- alpha/ gamma Antibody, Phosphatidylinositol 3 kinase catalytic alpha polypeptide Antibody, PI3K Antibody, PIK3R1 Antibody, PI3K alpha Antibody, PtdIns 3 kinase regulatory subunit alpha Antibody, Phosphatidyli |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthetic phospho-peptide from human PIK3R1 + PIK3R3 proteins around the phosphorylation site of PI3-kinase p85- alpha at tyrosine 467 (pTyr467) and PI3-kinase p85- gamma at tyrosine 199 (pTyr199). |
Immunogen Species: |
Human |
Applications: |
WB, ICC/IF |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human, Mouse, Rat |
Antibody Dilution: |
WB (1:250-1:1000); ICC/IF (1:50-1:250); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects endogenous levels of PI3-kinase p85- alpha only when phosphorylated at tyrosine 467 (pTyr467) and PI3-kinase p85- gamma only when phosphorylated at tyrosine 199 (pTyr199). Detects a band at ~55 kDa. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
N/A |
Cellular Localization: |
Cytosol, Endoplasmic Reticulum, Perinuclear Endoplasmic Reticulum Membrane, Nucleus, Plasma Membrane, Cell-Cell Junction, Cis-Golgi Network, Cytoplasm, Membrane, Phosphatidylinositol 3-Kinase Complex, Phosphatidylinositol 3-Kinase Complex Class Ia, P |
Tissue Specificity: |
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). Highest levels in brain and testis. Lower levels in adipose tissue, kidn |
Accession Number: |
NP_001229395.1/NP_001107644.1 |
Gene ID: |
5295/8503 |
Swiss-Prot: |
P27986/Q92569 |
Field of Use: |
For in vitro research use only. |