PINK1 Antibody | 7859

PINK1 Antibody | 7859 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1.
The immunogen is located within amino acids 120 - 170 of PINK1.

Research Area: Autophagy, Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: PINK1 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will only detect the longer isoform. PINK1 antibody will detect the cleaved and uncleaved form of PINK1.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 3: Cat. No. 1466 - Rat Spleen Tissue Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 55 kDa
Observed: 53 kDa

Validation: N/A

Isoform: N/A

Purification: PINK1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PINK1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PINK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: PINK1 Antibody: BRPK, PARK6BRPK

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1) . PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2) . PINK1 is cleaved by the mitochondrial protease PARL (3) . Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4) .