PIP5KI gamma (PIP5K1G) Antibody (C-term) [APR14373G]

PIP5KI gamma (PIP5K1G) Antibody (C-term) [APR14373G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PIP5KI gamma (PIP5K1G) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-668 amino acids from the C-terminal region of human PIP5KI gamma (PIP5K1G) .

Cellular Localisation: Cell membrane; Peripheral membrane protein; Cytoplasmic side {ECO:0000250|UniProtKB:Q5I6B8}. Endomembrane system {ECO:0000250|UniProtKB:Q5I6B8}. Cytoplasm {ECO:0000250|UniProtKB:O70161}. Cell junction, focal adhesion. Cell junction, adherens junction. Cell projection, ruffle membrane {ECO:0000250|UniProtKB:Q5I6B8}. Cell projection, phagocytic cup {ECO:0000250|UniProtKB:O70161}. Cell projection, uropodium {ECO:0000250|UniProtKB:O70161}. Note=Detected in plasma membrane invaginations. Isoform 3 is detected in intracellular vesicle-like structures

Molecular Weight: 73260

Clone: Polyclonal

Gene Name: PIP5K1C (HGNC:8996)

Gene ID: 23396

Function: Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns (4) P/PI4P) to form phosphatidylinositol 4, 5- bisphosphate (PtdIns (4, 5) P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed:12422219, PubMed:22942276) . PtdIns (4, 5) P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1, 4, 5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3, 4, 5-trisphosphate (PtdIns (3, 4, 5) P3/PIP3) (Probable) . PIP5K1A-mediated phosphorylation of PtdIns (4) P is the predominant pathway for PtdIns (4, 5) P2 synthesis (By similarity) . Together with PIP5K1A, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps (By similarity) . Promotes particle attachment by generating the pool of PtdIns (4, 5) P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport (By similarity) . Controls the plasma membrane pool of PtdIns (4, 5) P2 implicated in synaptic vesicle endocytosis and exocytosis (PubMed:12847086) . Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2) (PubMed:12847086) . Required for clathrin-coated pits assembly at the synapse (PubMed:17261850) . Participates in cell junction assembly (PubMed:17261850) . Modulates adherens junctions formation by facilitating CDH1/cadherin trafficking (PubMed:17261850) . Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions (PubMed:12422219) . Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins (PubMed:12422219) . Required for uropodium formation and retraction of the cell rear during directed migration (By similarity) . Has a role in growth factor-stimulated directional cell migration and adhesion (By similarity) . Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor (PubMed:17635937) . Negative regulator of T-cell activation and adhesion (By similarity) . Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor (By similarity) . Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (By similarity) .

Summary: Tissue Location: [Isoform 1]: Isoform 1 is strongly expressed in brain and also detected in heart and lung [Isoform 3]: Isoform 3 is detected in large amounts in heart and large intestine, is also present in lung, pancreas and thyroid, and to a lesser extent in brain, stomach and kidney

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma, PIP5K1-gamma, PtdIns (4) P-5-kinase 1 gamma, Phosphatidylinositol 4-phosphate 5-kinase type I gamma, PIP5KIgamma, PIP5K1C, KIAA0589