PKC ε (phospho-S729) polyclonal Antibody | BS4583

PKC ε (phospho-S729) polyclonal Antibody | BS4583 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB

Application Range: WB: 1:500~1:1000

Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, ∂, é, Ω, h, †, λ/î, µ and n) . Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Storage & Stability: Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.

Specificity: PKC ε (phospho-S729) polyclonal Antibody detects endogenous levels of PKC ε protein only when phosphorylated at Ser729.

Molecular Weight: ~ 76~85 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE

Immunogen: Synthetic phosphopeptide derived from human PKC ε around the phosphorylation site of Serine 729

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Contribution of Soluble Factors to EMT,