PKCepsilon, Active | 7753

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Protein kinase C epsilon (PRKCE), one of the PKC family members, has been shown to be involved in many different cellular functions, such as neuron channel activation (1), apoptosis (2), cardioprotection from ischemia (3, 4), heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior (5).

7753 | PKCepsilon Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKC, protein kinase C, epsilon

Alternates names: PKC, protein kinase C, epsilon

Taglines: Phosphorylate a wide variety of protein targets

NCBI Gene ID #: 18049

NCBI Gene Symbol: NGF

Gene Source: N/A

Accession #: P01139

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 1258 nmol phosphate incorporated into PKC substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: This NGF- is fully biologically active when compared to standards. The ED as measured by the dose-dependent induction of human TF-1 cell proliferation is typically 0.1–1 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 110.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml containing 1% HSA or BSA. The solution can be diluted into other aqueous buffers and stored at 4°C for 2-7 days or store at –20°C for future use.

Amino acid sequence: N/A