PKCnu, Active | 7746

PKCnu, also known as PKD3, is a members of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCnu is composed of 890 amino acid residues and the protein has 77.3% similarity to human PKC mu (PKCmu) and 77. 4% similarity to mouse PKD (the mouse homolog of PKCmu) (1). The PKCnu mRNA is ubiquitously expressed in various tissues and the gene is located between markers WI-9798 and D2S177 on chromosome 2p21 region.PKCnu has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCnu is trans-located to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCnu is an important physiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induced a rapid activation of PKCnu by a protein kinase C (PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCnu. PKCnu is present both in the nucleus and cytoplasm and this distribution of PKCnu results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induced a rapid and reversible plasma membrane translocation of PKCnu that is PKC-dependent.

7746 | PKCnu Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKC, Protein kinase C nu

Alternates names: PKC, Protein kinase C nu

Taglines: Involved in regulation of glycogen, sugar, and lipid metabolism

NCBI Gene ID #: 4908

NCBI Gene Symbol: NTF3

Gene Source: N/A

Accession #: P20783

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 177 nmol phosphate incorporated into CREBTIDE substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 142.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml.

Amino acid sequence: N/A