PKR Antibody | 3949

PKR Antibody | 3949 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Rat

Homology: Predicted species reactivity based on immunogen sequence: Mouse (86%)

Immunogen: Anti-PKR antibody (3949) was raised against a 14 amino acid synthetic peptide near the amino terminus of human PKR.
The immunogen is located within the first 50 amino acids of PKR.

Research Area: Innate Immunity

Tested Application: E, WB, IHC-P

Application: WB: 1-2 μg/mL; IHC-P: 5 μg/mL.
Antibody validated: Western Blot in human and mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1208 - THP-1 cell lysate

Positive Control 2: Cat. No. 1462 - Rat Lung Tissue Lysate

Positive Control 3: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4: Cat. No. 1202– A431 Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 62kD
Observed: 68kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar PKR expression profile in human and mouse cell lines detected by two independent anti-PKR antibodies that recognize different epitopes, 3947 against C-terminus domain and 3949 against N-terminus domain.  PKR proteins are detected in all the tested human cell lines except SK-N-SH cell line at different expression levels  by the two independent antibodies.  

Isoform: Human PKR has 2 isoforms, including isoform 1 (551aa, 62.1kD) and isoform S (510aa, 57.4kD. This antibody detects both human isoforms. Mouse PKR has 1 isoform (515aa, 58.3kD) . Rat PKR has only one isoform identified so far (513aa, 58.3kD) .

Purification: PKR Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PKR Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PKR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA) -dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.