PLC gamma 2 Antibody | 3710

PLC (Phosphoinositide-specific phospholipase C) plays a significant role in transmembrane signaling. Four members of PLCs have been identified: PLC?, PLCg, PLCd, and PLCe. In response to extracellular stimuli (e.g., hormone, growth factors, neurotransmitters), PLC hydrolizes phosphatidylinositol 4,5-biphosphate (PIP2) into two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PLCg2 is engaged in antigen-dependent signaling in B-cells and collagen-dependent signaling in platelets.

3710 | PLC gamma 2 Antibody DataSheet

Antibody Target: PLC gamma 2

Target Alternative Name: PLC2 , PLC148 , PLC-II , PLC-148, inositoltrisphosphohydrolase

Tag Line:

Category: PLC

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: Synthetic peptide surrounding amino acid 1252 of human PLCg2

Accession #: AAH18646

Gene ID: 5336

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot, Immunohistochemistry

Positive Control: Western Blot: Jurkat cell lysate . IHC: Brain and Cortex tissue

Application And Usages: Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 139 kDa human PLCg2. Jurkat cell lysate can be used as a positive control. Reactivity to other species has not been determined.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified: Not Verified

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified