Description
Pleckstrin Antibody | 46-201 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: C-EWIKAIQMASRTGK
Research Area: Cancer
Tested Application: E, WB, IF, Flow, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40-45kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1kDa according to Human NP_002655.2) . Recommended concentration: 0.01-0.3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 ( hepatocellular carcinoma) cell lysate.Immunohistochemistry:Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: PLEK, pleckstrin, P47, p47, FLJ27168
User Note: N/A
BACKGROUND: N/A