PLEKHM1 Antibody (N-term) [APR12896G]

PLEKHM1 Antibody (N-term) [APR12896G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PLEKHM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human PLEKHM1.

Cellular Localisation: Autolysosome membrane. Endosome membrane. Late endosome membrane. Lysosome membrane. Note=In case of infection colocalizes with Salmonella typhimurium sifA in proximity of Salmonella-containing vacuole (SCV) (PubMed:25500191) .

Molecular Weight: 117443

Clone: Polyclonal

Gene Name: PLEKHM1 (HGNC:29017)

Gene ID: 9842

Function: Acts as a multivalent adapter protein that regulates Rab7- dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Acts as a dual effector of RAB7A and ARL8B that simultaneously binds these GTPases, bringing about clustering and fusion of late endosomes and lysosomes (PubMed:25498145, PubMed:28325809) . Required for late stages of endolysosomal maturation, facilitating both endocytosis- mediated degradation of growth factor receptors and autophagosome clearance. Interaction with Arl8b is a crucial factor in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145) . Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity) . May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7 (PubMed:20943950) . May have a role in sialyl-lex- mediated transduction of apoptotic signals (PubMed:12820725) . Involved in bone resorption (By similarity) .

Summary: Tissue Location: Expressed in placenta, liver, prostate, thymus, spleen, ovary, colon, colon carcinoma and peripheral blood lymphocytes (PBL) . Weakly expressed in brain, lung, kidney, and testis. No expression in heart, skeletal muscle, pancreas and small intestine Predominantly expressed in the breast carcinoma cell line MCF-7

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Pleckstrin homology domain-containing family M member 1, PH domain-containing family M member 1, 162 kDa adapter protein, AP162, PLEKHM1, KIAA0356