PNKP Antibody (N-term) [APR31200G]

PNKP Antibody (N-term) [APR31200G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PNKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PNKP.

Cellular Localisation: Nucleus. Chromosome. Note=Localizes to site of double-strand breaks.

Molecular Weight: 57076

Clone: Polyclonal

Gene Name: PNKP {ECO:0000303|PubMed:10446192, ECO:0000312|HGNC:HGNC:9154}

Gene ID: 11284

Function: Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways (PubMed:10446192, PubMed:10446193, PubMed:15385968, PubMed:20852255, PubMed:28453785) . Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone (PubMed:10446192, PubMed:10446193) .

Summary: Tissue Location: Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192) . Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2' (3') -polynucleotidase, Polynucleotide 5'-hydroxyl-kinase, PNKP