Podoplanin (Human) ELISA Kit - E5051

E5051 | Podoplanin (Human) ELISA Kit DataSheet

Podoplanin, also known as PDPN is a small cell-surface mucin-like glycoprotein that plays a crucial role in the development of the alveoli, heart, and lymphatic vascular system. It mediates effects on cell migration and adhesion through its different partners. During development it plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation. PDPN has been studied extensively in the cancer field. It is a specific lymphatic vessel marker, and since lymphangiogenesis levels are correlated with poor prognosis in cancer patients. In squamous cell carcinomas, PDPN is believed to play a key role in the cancer cell invasiveness by controlling invadopodia, and thus mediating efficient ECM degradation. ’s Podoplanin (Human) ELISA Kit is used for the quantitative detection of Podoplanin in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of Podoplanin in the sample.

Alternate Name : PDPN ELISA Kit; Aggrus ELISA Kit; Gp38 ELISA Kit; RANDAM-2 ELISA Kit; T1A-2 ELISA Kit; 36-KD ELISA Kit; aggrus ELISA Kit; AGGRUS ELISA Kit; Gp36 ELISA Kit; GP40 ELISA Kit; HT1A-1 ELISA Kit; hT1alpha-1 ELISA Kit; hT1alpha-2 ELISA Kit; lung type I cell membrane associated glycoprotein ELISA Kit; lung type-I cell membrane-associated glycoprotein ELISA Kit; T1A-2 ELISA Kit; OTS 8 ELISA Kit; OTS8 ELISA Kit; PA2.26 antigen ELISA Kit; podoplanin ELISA Kit; T1A ELISA Kit; T1-alpha ELISA Kit

Tag Line : A highly sensitive ELISA kit for quantitative measurement of Podoplanin

Summary :

Detection Method : Absorbance is measured at 450 nm

Sample Type : Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity : Human

Applications : The ELISA kit is used for the quantitative detection of Podoplanin in serum, plasma, tissue homogenates and other biological fluids

Features and Benefits : Highly sensitive and specific Detection range: 0.156-10 ng/ml Sensitivity: 0.094 ng/ml Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Recovery Rate: 87-99% for Serum, 88-96% for EDTA plasma and 88-100% for Heparin plasma