Description
POLL Antibody | 46-207 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: C-LGLPYREPAERDW
Research Area: Cancer
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1) . Recommended concentration: 0.5-1ug/ml.
Specificiy: It has been brought to our attention that this product may crossreact with Pol beta (NP_002681) , an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
Positive Control 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: POLL, BETA-N, POL-KAPPA, polymerase (DNA directed) , lambda, polymerase (DNA-directed) , lambda, DNA polymerase kappa (DNA polymerase beta-N) , RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N) , DNA polymerase lamda2, DNA-directed DNA polymerase lambda, OTTHUMP00000020323, pol beta2, pol lambda
User Note: N/A
BACKGROUND: N/A