POU2AF1 Antibody [AMM02477G]

POU2AF1 Antibody [AMM02477G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: Purified His-tagged POU2AF1 protein was used to produced this monoclonal antibody.

Cellular Localisation: Nucleus.

Molecular Weight: 27436

Clone: Monoclonal

Gene Name: POU2AF1 (HGNC:9211)

Gene ID: 5450

Function: Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290) . It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176) . It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176) . It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290) . Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity) .

Summary: Tissue Location: B-cell specific (PubMed:7779176, PubMed:7859290) . Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290)

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:4000

Synonyms: POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1