PP2A alpha Antibody (C-term) [APR06087G]

PP2A alpha Antibody (C-term) [APR06087G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This PP2A alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the C-terminal region of human PP2A alpha.

Cellular Localisation: Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025) . During mitosis, also found at spindle poles (PubMed:16541025) . Centromeric localization requires the presence of SGO2 (By similarity) {ECO:0000250|UniProtKB:P63330, ECO:0000269|PubMed:16541025}

Molecular Weight: 35594

Clone: Polyclonal

Gene Name: PPP2CA

Gene ID: 5515

Function: PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (PubMed:22613722) . PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (PubMed:22613722) . Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity) . Can dephosphorylate SV40 large T antigen and p53/TP53 (PubMed:17245430) . Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed:10801873) . Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed:33108758) . Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed:25438055) . Mediates dephosphorylation of FOXO3; promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (PubMed:30513302) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA