PPAR (LBD) Human Recombinant (His-tagged) | 7878

The peroxisome proliferator activated receptor (PPAR) family of ligand-activated transcription factors consists of three subtypes encoded by separate genes: PPAR, PPAR and PPAR. Of these, PPAR plays an important role in the regulation of fatty acid storage and glucose metabolism. The genes activated by PPAR stimulate lipid uptake and adipogenesis by fat cells. Many naturally occurring molecules such as, polyunsaturated fatty acids like arachidonic acid and arachidonic acid metabolites, are known to bind and activate PPAR. The binding of activating ligands to the ligand binding domain (LBD) of PPAR promotes its heterodimerization with retinoic acid-like receptor (RXR), which results in the regulated expression of target genes involved in lipid metabolism. ’s His-PPAR (LBD) is a non-glycosylated protein expressed and purified from E. coli. It has been tested for functionality by its binding and subsequent displacement of 1,8-ANS assay.

7878 | PPAR LBD Human Recombinant His-tagged DataSheet

Biomolecule/Target: PPAR

Synonyms: PPAR-gamma, Peroxisome Proliferator-activated Receptor Ligand Binding Domain, PPAR-gamma (LBD), NR1C3, PPARG (LBD)

Alternates names: Activin beta-B, Inhibin beta-2

Taglines: Recombinant Human PPAR (LBD) can be used in the ligand screening assays, selectivity profiling, western blotting, ELISA, and numerous such applications

NCBI Gene ID #: 91

NCBI Gene Symbol: ACVR1B

Gene Source: Human

Accession #: P36896

Recombinant: Yes

Source: (BTI-Tn-5B1-4) Hi-5 Insect cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is 2.0 ng/ml, corresponding to a specific activity of 5 x 105 units/mg.

Biological activity: Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is 2.0 ng/ml, corresponding to a specific activity of 5 x 105 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25.6 kDa

Concentration: N/A

Appearance: Liquid. In 50 mM Tris, 100 mM NaCl, 1 mM TCEP, pH 8.0 containing 20% glycerol

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 3.0 and 5% Trehalose.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in 10 mM Sodium citrate, pH 3.0 to a concentration of 0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECGCA