PPP1CA protein (His tag) | 80R-1935

PPP1CA protein (His tag) | 80R-1935 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Conjugated Proteins

Research Area: Differentiation & Development

Short Description: Recombinant human PPP1CA protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 1-330 amino acids: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY  DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP  IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV  VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK

Tag/conjugate: His tag

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 80% pure

Methode of Purification: N/A

Source: N/A

Concentration: 0.25 mg/ml

Form & Buffer: Liquid. 50mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2

Storage: Can be stored at 4 deg C  short term (1-2 weeks) . For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: Specific activity: >3, 000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Usage Recommendations: PPP1CA protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user.

Assay Information: 1. Prepare a 200ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP. 2. Pre-warm reaction mix in heat block at 37C. 3. Add recombinant PPP1CA solution with various concentrations (2ug, 3ug) in 200ul reaction buffer. 4. Mix by inversion and incubate at 37C for 10minutes. 5. Stop the reaction by adding the 800ul of 0.1N NaOH. 6. Record the absorbance at 405nm