PR-1 | Positive control/quantitation standard | AS10 687S

PR-1 | Positive control/quantitation standard | AS10 687S | Gentaur UK, US & Europe Distribution

Immunogen: N/A

Host: N/A

Conjugation: N/A

Clonality: N/A

Isotype: N/A

Purity: N/A

Format: Lyophilized in glycerol.

Tested Application: Western blot (WB)

Related Products: AS10 687 | Anti-PR-1 | Pathogenesis-related protein 1, rabbit antibodiesCollection of antibodies to other proteins involved in a response to pathogen attackPlant protein extraction buffer

Recommended Dilutions: Standard curve: 3 loads are recommended eg.0.5, 2 and 4µl.For most applications a sample load of 10-20 µg of protein will provide with a signal in this range.Positive control:a 2µl load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Molecular weight: 16, 4 kDa

Confirmed Reactivity: N/A

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: Concentration: after adding 90 µl of sterile milliQ water final concentration of the standard is 0.10 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Background: Pathogenesis-related protein 1 (PR-1) is partially responsible for acquired pathogen resistance. Induced by INA, salicylic acid and pathogen infection.This product is a recombinant PR-1 protein, trunctated by first 26 amino acids, source: Arabidopsis thaliana, UniProt: P33154, TAIR: At2g14610

Reconstitution: For reconstitution add 90 µl of sterile water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: At2g14610

Category: Pathogen attack

Research Area: Plant Biology, Pathogens