PR-3 / CHN | Class I chitinase | AS07 207

PR-3 / CHN | Class I chitinase | AS07 207 | Gentaur UK, US & Europe Distribution

Immunogen: Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992) : 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein G purified in PBS pH 7.4.

Format: Lyophilized

Tested Application: Co-Immunoprecipitation (IP) (Co-IP), Immunolocalization (IL), Western blot (WB)

Related Products: AS10 687 | Anti-PR-1 | Pathogenesis-related protein 1, rabbit antibodies AS12 2366 | Anti-PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thalianaAS07 208 | Anti-PR-2 | GLU I | class I beta-1, 3-glucanase, rabbit antibodies for other species, not Arabidopsis thalianaAS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodiesAS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodiescollection of antibodies to other proteins involved in a response to pathogen attackSecondary antibodies

Recommended Dilutions: 8 µg/ml (WB)

Molecular weight: 35, 34 | 32 and 34 kDa

Confirmed Reactivity: Agostis stolonifera cv. 'Penncross', Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera

Predicted Reactivity: Arabidopsis thaliana, Manihot esculenta, Zea maysSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Important note: For blocking 5 % skim milk in PBS without Ca++ should be used, This antibody is purified by affinity chromarography on Portein G

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Pathogen attack

Research Area: Plant Biology