PRDM16 Antibody | 5555

PRDM16 Antibody | 5555 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: PRDM16 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16.
The immunogen is located within amino acids 1120 - 1170 of PRDM16.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 109, 120, 140 kDa
Observed: 140 kDa

Validation: N/A

Isoform: N/A

Purification: PRDM16 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PRDM16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PRDM16 Antibody: MEL1, LVNC8, PFM13, CMD1LL, KIAA1675, MEL1, PR domain zinc finger protein 16, PR domain-containing protein 16

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PRDM16 Antibody: PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t (1;3) (p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) . This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t (1:3) (p36, q21) -positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.